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Autori: ZVEZDELINA YANEVA, NEDYALKA GEORGIEVA.
DOI: https://doi.org/10.24193/subbchem.2017.2.15
Published Online: 2017-06-20
Published Print: 2017-06-30
VIEW PDF: A SENSITIVE ANALYTICAL (RP-HPLC-PDA, UV/VIS) METHOD…
The purpose of the present study was to develop a simple, rapid and reproducible analytical method for the determination of N-isonicotynoyl-Nˊ-(3-fluorobenzal)hydrazone (SH2) - a synthesized by us halogenated isoniazid derivative with high tuberculostatic activity, in aqueous phase, on the basis of RP-HPLC-PDA and UV/VIS spectrophotometric investigations. Despite of the high linearity (R2 0.9984) of the UV/VIS spectrophotometric method applied, the significantly higher LOQ and LOD values indicated its unsuitability for detection and quantification of low N-isonicotynoyl-Nˊ-(3-fluorobenzal)hydrazone concentrations (< 10 µg/mL). The proposed RP-HPLC-PDA method with mobile phase ACN/phosphate buffer (60:40, v/v) offered short retention time (3.1 min), high precision (RSD 3.50 %) and linearity (R2 0.9898). It characterized with satisfactory LOD (0.346 µg/mL) and LOQ (1.05 µg/mL) values and allowed the qualitative detection of SH2 E/Z-isomer.
Keywords: N-isonicotynoyl-Nˊ-(3-fluorobenzal)hydrazone, RP-HPLC-PDA, UV/VIS, aqueous phase
